The effect of pseudomonas exotoxin A on cytokine production in whole blood exposed to Pseudomonas aeruginosa |
| |
Authors: | Schultz M J Speelman P Zaat S A Hack C E van Deventer S J van der Poll T |
| |
Affiliation: | Department of Infectious Diseases, Tropical Medicine and AIDS, Academic Medical Center, University of Amsterdam, The Netherlands. m.j.schultz@amc.uva.nl |
| |
Abstract: | To determine the effect of Pseudomonas aeruginosa exotoxin A (P-ExA) on cytokine production, we studied cytokine release induced by heat-killed P. aeruginosa (HKPA) in human whole blood in the presence or absence of P-ExA. P-ExA (0.01-1 microgram ml(-1)) caused a dose-dependent decrease in HKPA-induced production of tumor necrosis factor alpha (TNF), interleukin (IL-) 10, IL-6 and IL-8 (all P<0.05). P-ExA-induced inhibition of IL-10, IL-6 and IL-8 release was not dependent on reduced TNF concentrations, since the relative attenuation of the production of these cytokines was similar in the presence or absence of a neutralizing anti-TNF antibody. The effect of P-ExA on cytokine production may offer a disadvantage to the host with respect to clearance of the infection. |
| |
Keywords: | Cytokine Host defense Pseudomonas aeruginosa |
本文献已被 PubMed 等数据库收录! |
|