Active transport of alpha-aminoisobutyric acid in freshly prepared rat hepatocytes |
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Authors: | Chi-Po Chen Reynolds H Lee |
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Institution: | Section of Pharmacology and Toxicology School of Pharmacy University of Connecticut Storrs, Connecticut 06268, USA |
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Abstract: | The transport of alpha-aminoisobutyric acid in freshly prepared rat liver cells was saturable and exhibited a Kt of 13.9 × 10?3M and amax of 28.6 umoles/ml intracellular fluid/30 min. The system required the presence of sodium and was sensitive to ouabain. Anaerobiosis, 2,4-dinitrophenol and low temperature suppressed the uptake of the amino acid. Efflux studies also indicated that the majority of the intracellular amino acid was rapidly exchangeable and therefore probably present in the cell water in a free state. It is suggested that alpha-aminoisobutyric acid is transported into isolated rat hepatocytes by an active carrier system. |
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