Chemiluminescence associated with phagocytosis of foreign particles in rabbit alveolar macrophages |
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Authors: | PR Miles Ping Lee Michael A Trush Knox Van Dyke |
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Institution: | 1. Department of Physiology & Biophysics and Pharmacology West Virginia University Medical Center, USA;2. Appalachian Laboratory for Occupational Safety and Health Morgantown, West Virginia 26506, USA |
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Abstract: | Rabbit alveolar macrophages exhibit a chemiluminescent response which is associated with phagocytosis of zymosan and polystyrene-butadiene particles. The chemiluminescence reaches a peak in 15 to 25 minutes and then gradually diminishes over the next 1 to 3 hours. During the time of maximal light emission there appears to be no actual uptake of particles, but the response is dependent upon the particle concentration. The metabolic inhibitor, DNP (2,4-dinitrophenol), causes a rapid inhibition of the chemiluminescent response. The addition of ATP to the medium prior to exposure of the cells to particles causes the chemiluminescent response to be greatly diminished, i.e., 0.3mM ATP virtually abolishes the response. These experiments suggest that some metabolic response of the cell to phagocytosis is responsible for the chemiluminescence. |
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