Localisation and movement of Plum pox virus in apricot stem tissues |
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Authors: | F DICENTA ,P MARTÍ NEZ-GÓ MEZ,M RUBIO,J M AUDERGON |
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Affiliation: | Departamento de Mejora y Patología Vegetal, CEBAS-CSIC, Apartado 4195, E-30080 Murcia, Spain;Unitéde Génétique et d'Amélioration des Fruits et Légumes - Institut National de la Recherche Agronomique, Site AGROPARC, Domaine Saint Paul, BP91, F-84914 Avignon, Cedex 9, France |
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Abstract: | Localisation and movement of Plum pox virus (PPV), sharka disease, in stem tissues of susceptible and resistant apricot (Prunus armeniaca L.) cultivars was studied. Two different assays were performed. In the first assay, apricot cultivars were grafted on to a non‐inoculated GF305 peach rootstock and, after two months, the sprouted apricot was inoculated by chip‐grafting. In the second assay, apricot cultivars were grafted on to a previously chip‐inoculated GF305 showing strong PPV symptoms. Localisation of virus was studied in apricot stem by immuno‐tissue printing and sharka symptoms in GF305 and apricot leaves were also observed. Virus was mainly localised in the xylem, and sometimes in the cortex and pith. Results revealed that, while all the cultivars allowed limited virus movement from the inoculation point, only the susceptible cultivars (Screara, Bebeco and Colomer) allowed long distance movement and even showed symptoms in leaves. |
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Keywords: | Prunus armeniaca L Plum pox virus virus long-distance movement immuno-tissue printing |
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