Identification of the development stage—specific factors in mouse fetal liver binding to the human β—globin gene promoter |
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引用本文: | CHENYADI YULONGHU 等.Identification of the development stage—specific factors in mouse fetal liver binding to the human β—globin gene promoter[J].细胞研究,1994,4(1):9-15. |
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作者姓名: | CHENYADI YULONGHU |
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作者单位: | [1]ShanghaiInstituteofCellBiology,AcademiaSinicaShanghai200031,China. [2]ShanghaiInstituteofCellBiology,Aca |
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摘 要: | In order to elucidate the molecular mechanisms of globin gene expression during embryonic development,the nuclear extracts from mouse hematopoietic tissue at different stages of development have been prepared.By using DNase I footprinting and gel mobility shift assays,the binding of protein factors in these extracts to the human β-globin promoter was analyzed.The differences in the binding patterns of protein factors during development were observed.An erythroid-specific and stage-specific nuclear protein in the nuclear extrace from d 18 mouse fetal liver was identified,which can bind to the sequence(from-66bp to-90bp) of human β-globin promoter.We therefore speculate that the function of this cis-acting element may be similar to stage selector element(SSE) in chicken β^A-promoter.
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关 键 词: | 人β-珠蛋白基因 启动子 结合 反式作用元件 鼠 胎肝 发育阶段特异性因子 鉴定 孕期 调控 |
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