X-linked heterochromatin distribution in the holocentric chromosomes of the green apple aphid <Emphasis Type="Italic">Aphis pomi</Emphasis> |
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Authors: | A?Criniti G?Simonazzi S?Cassanelli M?Ferrari D?Bizzaro Email author" target="_blank">G?C?ManicardiEmail author |
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Institution: | (1) Dipartimento di Scienze Agrarie, Università di Modena e Reggio Emilia, Reggio Emilia, Italia;(2) Istituto di Biologia e Genetica, Università Politecnica delle Marche, Ancona, Italia |
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Abstract: | Chromatin organization in the holocentric chromosomes of the green apple aphid Aphis pomi has been investigated at a cytological level after C-banding, NOR, Giemsa, fluorochrome staining and fluorescent in situ hybridization (FISH). C-banding technique showed that heterochromatic bands are exclusively located on X chromosomes. This data represents a peculiar feature that clearly contradicts the equilocal distribution of heterochromatin typical of monocentric chromosomes. Moreover, silver staining and FISH carried out with a 28S rDNA probe localized rDNA genes on one telomere of each X chromosome; CMA3 staining reveals that these silver positive telomeres are the only GC-rich regions among A. pomi heterochromatin, whereas all other C-positive bands are DAPI positive thus containing AT-rich DNA. |
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Keywords: | A pomi AgNORs aphid cytogenetics heterochromatin holocentric chromosomes |
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