A comparison between two methods of extracting chlorophyll-a from different phytoplankton samples |
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Authors: | R. M. M. Roijackers |
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Affiliation: | (1) Laboratory of Hydrobiology, Agricultural University, De Dreijen 12, 6703 BC Wageningen, The Netherlands |
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Abstract: | Summary A comparison has been made between chlorophyll-a estimations based on the cold acetone extraction procedure and on the hot ethanol extraction method. When comparing the results obtained with both methods a standard deviation of 20% must be taken into account. A correlation between extraction efficiency and phytoplankton species composition could not be found. Although the chlorophyll-a yield in ethanol extracts is not better than that in acetone extracts, there are several advantages in using the ethanol method, particularly the short extraction period and the cheaper procedure. |
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