| 猪瘟病毒C株兔脾毒在SK6细胞中的增殖培养及其鉴定 |
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| 引用本文： | 张淼涛,冯霞,刘湘涛,张彦明,谢庆阁.猪瘟病毒C株兔脾毒在SK6细胞中的增殖培养及其鉴定[J].Virologica Sinica,2004,19(4):404-406. |
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| 作者姓名： | 张淼涛 冯霞 刘湘涛 张彦明 谢庆阁 |
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| 作者单位： | [1]中国农业科学院兰州兽医研究所畜禽疫病重点实验室，甘肃兰州730046 [2]西北农林科技大学，陕西杨凌，710021 |
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| 基金项目： | 国家重点基础研究发展规划(G19990119) |
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| 摘 要： | Enzyme-linked immunosorbent assay(ELISA), direct florescent antibody staining, and RT-PCT were used to detect growth characteristics of Cassical swine fever virus C-strain (Derived from Spleen) in SK6 cell. The results indicated than C-strain (Derived from Spleen) can grow in SK6 cell at a lower level. Direct florescent antibody staining method was not suitable for the detection of attenuated lapinized C-strain. The study provided a primary guide for the detection of attenuated classical swine fever virus. It also supplies an elementary foundation for the study of its growth characteristic in SK6.
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| 关 键 词： | 猪瘟病毒 CSFV C株 增殖培养 鉴定 变异 |
The Culture Characteristics of Classical swine fever virus C-strain(Derived from Spleen) in SK6 Cell and Its Detection |
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| Lanzhou Veterinary Research Institute,Lanzhou ,China,.North-West Sci-Tech University of Agriculture and Forestry,Yanling ,China.The Culture Characteristics of Classical swine fever virus C-strain(Derived from Spleen) in SK6 Cell and Its Detection[J].中国病毒学(英文版),2004,19(4):404-406. |
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| Authors: | Lanzhou Veterinary Research Institute Lanzhou China North-West Sci-Tech University of Agriculture and Forestry Yanling China |
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| Institution: | Lanzhou Veterinary Research Institute,Lanzhou 730046,China,2.North-West Sci-Tech University of Agriculture and Forestry,Yanling 710021,China) |
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| Abstract: | Enzyme-linked immunosorbent assay(ELISA), direct florescent antibody staining, and RT-PCT were used to detect growth characteristics of Cassical swine fever virus C-strain (Derived from Spleen) in SK6 cell. The results indicated than C-strain (Derived from Spleen) can grow in SK6 cell at a lower level. Direct florescent antibody staining method was not suitable for the detection of attenuated lapinized C-strain. The study provided a primary guide for the detection of attenuated classical swine fever virus. It also supplies an elementary foundation for the study of its growth characteristic in SK6. |
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| Keywords: | Classical swine fever virus C-strain Multiplication culture Detection |
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