Chemical characterization of exopolysaccharides from the marine fouling diatom amphora coffeaeformis |
| |
Authors: | Bhosle N B Sawant S S Garg A Wagh A B Evans L V |
| |
Affiliation: | a Marine Corrosion and Materials Division , National Institute of Oceanography , Dona Paula , Goa , 403004 , India Fax: |
| |
Abstract: | Amphora coffeaeformis cells were grown in batch cultures under continuous illumination at 18°C for 10 d. Algal cells were removed by centrifugation, lyophilized and used for the extraction of exopolysaccharides using either 0.05 M EDTA, 1 M NaOH or 1.5 M NaCl. The 1.5 M NaCl treatment removed most exopolysaccharides. Glucose (81%) was the most abundant monosaccharide in the exopolysaccharides. The chemical composition data suggest that the exopolymers were acidic sulphated polysaccharides containing high concentrations of pyruvate (22%) and uronic acids (18%). These polysaccharides may play an important role in metal complexation and protection from desiccation in A. coffeaeformis. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|