中医病证相符的糖尿病慢性皮肤溃疡大鼠造模方法初探

目的观察不同造模方法致糖尿病大鼠慢性皮肤溃疡创面形态及愈合时间的影响。方法 50只SD大鼠随机分为5组:皮肤缺损组(QS组:剪皮),糖尿病组(DM组:STZ+剪皮),糖尿病加金黄色葡萄球菌组(DMJJ组:STZ+剪皮+金葡菌),糖尿病加激素组(DMJS组:STZ+剪皮+激素注射),糖尿病加激素加异物组(DMYW组:STZ+剪皮+激素注射+异物埋置)。糖尿病模型稳定后每周测量血糖1次,每日称量体重、观察疮面情况、测量创面面积。12 d后处死,石蜡包埋肉芽组织观察其组织病理形态。结果 DMJJ组前5d愈合速度快于其余组(P0.01);DMYW组的愈合时间延长,DMYW组愈合率显著偏低,与其余组比较有统计学意义(P0.01)。造模12 d其余组愈合率无统计学差异,DMYW组愈合率显著低于其余组(P0.01)。结论注射激素大鼠表现出中医"阴证"证型特点,糖尿病加激素注射加异物埋置复合因素造模法能使大鼠创面表现出与临床相似的"阴证"证型特点。

Exploration of the establishment of a rat model of chronic diabetic skin ulcer simulating the traditional Chinese syndrome

Objective To observe the effects of different modeling methods on the wound morphology and healing time of chronic skin ulcers in diabetic rats. Methods SD rats （n=50） were randomly divided into five groups, 10 rats in each group： skin defect group （group QS： dermal deficiency）, diabetes group （group DM： STZ injection＋skin excision）, diabetes plus Staphylococcus aureus group （group DMJJ： STZ injection＋skin excision＋bacterial infection）, diabetes plus hydrocortisone group （group DMJS： STZ injection＋skin excision＋hydrocortisone intervention） and diabetes plus hydrocortisone and implantation of foreign body group （DMYW group： STZ injection＋skin excision＋hydrocortisone intervention ＋ foreign body embedded）. The rats were measured for body weight and wound healing every day, and blood glucose after stable diabetes once a week. The rats were sacrificed 12 days later and the skin lesions were examined by histopathology.Results The healing rate of the DMYW group was significantly slower than that in the other groups （P〈0.01）. At 12 days after modeling, the healing rate of the DMYW group was significantly lower than that of the remaining groups （P〈0.01）, while the healing rates were not significantly different among the remaining groups.Conclusions The modeling method of DMYW group can show skin wounds similar to the clinical characteristics of ＂Yin syndrome＂, and the addition of foreign body implantation significantly prolongs the rat skin healing time.