七个中药复方对小鼠止咳化痰作用的对比实验

目的为了筛选出防治猪呼吸道疾病综合征(PRDC)有效的中药复方制剂,对七个具有止咳化痰作用的中药复方制剂进行了对比实验。方法以小鼠为实验动物,用右美沙芬、氯化铵作阳性药,生理盐水作空白对照,采用浓氨水引咳法和气管段酚红分泌法,对七个中药复方制剂的止咳化痰作用进行了观测。结果方7、方5可显著延长小鼠的咳嗽潜伏期(P0.05),减少5 min内的咳嗽次数(P0.05),除方4组外,其他中药组的气管酚红排泌量显著低于对照组(P0.05),并且方7、方5和氯化铵组小鼠的酚红排泌量显著低于其他处理组(P0.05)。结论七个中药复方组中,方5、方7的止咳化痰作用最显著,可在进一步实验研究后,作为PRDC的治疗药物。

Experimental comparison of the antitussive and expectorant effects of seven traditional Chinese medicine compounds in mice

Objective In order to screen effective traditional Chinese medicine compounds to prevent and control porcine respiratory disease syndrome （ PRDC） , seven compound preparations of traditional Chinese medicine were tested and to analyze their antitussive and expectorant effects in mice .Methods Two hundred 6-week old ICR mice （ male∶fe-male=1∶1） were used in this study .Dextromethorphan and ammonium chloride were used as positive control drugs , and physiological saline was used as blank control .The antitussive and expectorant effects of the seven Chinese medicine com-pounds （groups 1, 2, 3, 4, 5, 6, 7） were observed by ammonia-induced cough model and tracheal phenol red secretion method in mice .Results The results showed that compounds 7 and 5 significantly prolonged the cough incubation period （P 〈0.05）, and reduced the cough times within 5 min （P 〈0.05）.Except for the group 4, tracheal phenol red excre-tion in the other groups was significantly lower than that of blank control group （P 〈0.05）, and phenol red excretion in the mice of groups 7, 5 and ammonium chloride group was significantly lower than that in other treatment groups （ P 〈0.05）.Conclusions The Chinese medicine compounds 5 and 7 show most evident expectorant effects , and worthy of fur-ther validation of them as a drug in the treatment of porcine respiratory disease syndrome .