Trichuris suis: detection of antibacterial activity in excretory-secretory products from adults. |
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| Authors: | S R Abner G Parthasarathy D E Hill L S Mansfield |
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| Institution: | National Food Safety and Toxicology Center, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan 48824, USA. |
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| Abstract: | Antibacterial activity was detected in excretory-secretory products (ESP) of adult Trichuris suis cultured in vitro in serum-free media. Gram-negative bacteria (Campylobacter jejuni, Campylobacter coli, and Escherichia coli) and Gram-positive bacteria (Staphylococcus aureus) were sensitive to ESP. Susceptibility was dependent on the concentration of ESP but not on the inoculum size. Preliminary assessment of the mode of action suggests a bacteriocidal mechanism. This antibacterial activity was heat stable and resistant to digestion with pronase E and trypsin. Based on ultrafiltration experiments, the activity is less than 10,000 MW. This excreted/secreted antibacterial activity from T. suis is likely a component of a humoral defense system for this helminth. |
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