| Abstract: | The purpose of this study was to determine whether samples from the veins of the pregnant and the nonpregnant horn of the uterus lead to similar estimates of uterine blood flow and oxygen consumption. To accomplish this, a comparison of uterine blood flow, arteriovenous differences of oxygen content, and oxygen consumption measured by sampling the venous drainages of the two uterine horns was performed on eight pregnant sheep during the last 20 days of pregnancy. Each sheep carried a single fetus. Umbilical and uterine blood flows were measured with the test substances ethanol and antipyrine by application of the steady-state diffusion method. Twenty-three measurements of uterine blood flow comparing the two horns were not significantly different (P greater than 0.1), and were highly correlated (r = 0.98). The ratio of the oxygen content arteriovenous difference in the pregnant to that in the nonpregnant horn and the ratio of the uterine blood flow in the nonpregnant to that in the pregnant horn were significantly correlated (r = 0.7). As a consequence, paired calculations of oxygen consumption for the whole pregnant uterus had a small coefficient of variation (+/- 3.7%). These results demonstrate that the use of highly diffusible test substances for the measurement of uterine blood flow in pregnant sheep can provide accurate data for the calculation of uterine oxygen uptake, in part because the oxygen and test substance molecules are similarly affected by local variations in placental perfusion. |
