Modulation of nonspecific defence mechanisms and specific immune responses after suppression induced by xenobiotics |
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| Authors: | By M Studnicka A K Siwicki M Morand A Rymuszka A Bownik E Terech-Majewska |
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| Institution: | Department of Biology and Toxicology, Catholic University of Lublin, Poland;;Fish Pathology and Immunology Research Laboratory, Inland Fisheries Institute, Poland;;Department of Infectious Diseases, Veterinary Faculty, University of Agriculture, Olsztyn, Poland;;Laboratoire Departemental d'Analyses, Conseil General du Jura, France |
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| Abstract: | Summary In the present study the possible modulation of nonspecific defence mechanisms and specific immune responses after suppression induced by oxytetracycline, oxolinic acid, and lindane – an organochlorine pesticide, were analysed in carp. Modulation was attempted using a natural immunostimulant, dimerized lysozyme (KLP-602, Nika Health Products, USA). Five groups of fish were given intraperitoneal injections of selected doses of the drugs on days 7, 6 and 4 before immunization with Yersinia ruckeri vaccine. At each injection, group I was also given 10 mg kg−1 oxytetracycline, group II 10 mg kg−1 oxolinic acid, group III 10 mg kg−1 lindane, group IV was only immunized, and group V was injected with PBS. The immunostimulant was added in food before and after immunization. The study showed the immunotoxic effects of oxytetracycline, oxolinic acid and lindane; dimerized lysozyme corrected the defence mechanisms suppressed by these drugs and chemicals. The immunostimulatory effects of dimerized lysozyme were better when added before rather than after fish immunization. |
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