Local Base Order Influences the Origin of ccr5 Deletions Mediated by DNA Slip Replication |
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Authors: | Chi-Yu Zhang Ji-Fu Wei Shao-Heng He |
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Institution: | (1) Allergy and Inflammation Research Institute, Medical College of Shantou University, Shantou, Guangdong, 515031, P.R. China;(2) Department of Biochemistry and Molecular Biology, School of Medical Technology, Jiangsu University, Zhenjiang, Jiangsu, 212001, P.R. China |
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Abstract: | CCR5 is a seven-transmembrane G-protein-coupled receptor that binds the CC-chemokines including RANTES, eotaxin, MIP-1α and
β. CCR5 serves as an essential coreceptor for cell entry of R5 (macrophage-tropic, nonsyncytium-inducing) strains of HIV-1.
To date, four deletions have been found in human and primate ccr5. There is little evidence, however, on how these deletion mutations occur. In the present study, we analyzed ccr5 sequences of both mutants and wild type and found that direct repeats flanked the breakpoints of the deletions, suggesting
that these deletions resulted from slipped mispairing during DNA replication. Of particular interest was the location of these
deletions in or near the regions with higher negative FORS-D values. High negative FORS-D values stand for high stem-loop
potential determined by base order and influence mainly the formation of stem-loop structures. Therefore, the particular location
of these deletions suggests that the local sequence of bases might be important in the initiation of deletions mediated by
DNA slip replication in concert with direct repeats.
Contributed to this paper equally |
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Keywords: | ccr5 deletion direct repeats stem-loop potential slip replication |
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