仙客来组织培养中再生器官类型及增殖稳定性比较

对‘胜利女神’(Cyclamen persicum cv. Victoria’)和‘大红’(C. persicum cv. ‘Dahong’)两个仙客来品种成苗叶片诱导的愈伤组织，在6-BA与NAA配合的12个分化培养基上继代培养，第一次继代主要产生大量的不定芽芽点，从第二次继代后会得到5种类型的再生器官，分别为不定芽芽点、多芽球茎、多芽新梢、单芽球茎和叶片状芽丛。通过对不同再生器官增殖稳定性的试验表明，‘大红’品种的多芽球茎和多芽新梢在继代培养中再生相同器官的稳定性比较强，由于多芽球茎的增殖率大于多芽新梢，在继代中选择多芽球茎为再生体系较理想；不定芽芽点在以后的继代中会分散产生各种再生器官，不宜作为增殖器官；单芽球茎和叶片状芽丛继代分散性强，为不正常再生器官。‘胜利女神’品种多芽球茎和多芽新梢继代的稳定性比‘大红’品种差，易于产生多种不正常器官。

Regenerated Organs of Cyclamen persicum Mill. and Their Proliferative Stability in Tissue Culture

Leaf calli of two cyclamen cultivars, ‘Victoria’ and ‘Dahong’ were inoculated on 12 kinds of media supplemented with of 6-BA and NAA. Five types of regenerated organs appeared, including adventitious bud point, multiple-bud bulb, multiple-leaf new shoot, single-bud bulb and leaf-like bud clump. Tests were made on proliferative stability of different regenerated organs and multiple-bud bulbs and multiple-leaf new shoost of ‘Dahong’ were more stable in continued culture. Compared with multiple-leaf new shoots, multiple-bud bulbs showed high proliferative rate and were ideal forcontinued culture. Single-bud bulbs and leaf-like bud clumps were abnormal regenerated organs. Multiple-bud bulbs and multiple-leaf new shoots of ‘Victoria’ were instability in continued cultureand they were liable to produce abnormal organs.