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Morphology and Antigenic Properties of Recombinant Analogs of the Marburg Virus Nucleoprotein |
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| Authors: | Kachko A V Cheusova T B Sorokin A V Kazachinskaya E I Cheshenko I O Belanov E F Bukreev A A Ivanova A V Razumov I A Ryabchikova E I Netesov S V |
| Institution: | (1) Institute of Molecular Biology, VECTOR State Research Center of Virology and Biotechnology, Kol'tsovo, Novosibirsk Region, 630559, Russia;(2) National Institute of Allergy and Infectious Diseases, National Cancer Institute, Bethesda, Maryland, United States |
| Abstract: | The full-length gene for Marburg virus (MV) nucleoprotein (NP) was cloned in prokaryotic pQE32 under the control of the T5 promoter and in eukaryotic pTM1 under the control of the T7 RNA polymerase promoter. Recombinant NP was synthesized in Escherichia coliand in human kidney cell line 293 cotransfected with recombinant vaccinia virus vTF7-3 expressing T7 RNA polymerase. On evidence of electron microscopy with immune detection, recombinant NP formed tubules of two types in E. coliand of a single type in cell line 293. ELISA and immunoblotting with polyclonal and monoclonal antibodies revealed common antigenic determinants in recombinant NP and natural MV NP. |
| Keywords: | filovirus Marburg virus nucleoprotein recombinant protein expression system morphology |
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