基因疫苗导入技术研究进展

基因疫苗积极的临床结果证明了,基因免疫是一种有效的临床免疫模式。虽然,喷射注射法的精确作用机制还不太清楚,但临床前研究表明,在皮肤内直接打靶抗原呈递细胞可有效地增强免疫反应。另外,局部给药法和树突细胞体外加载抗原的实验结果显示,直接打靶抗原呈递细胞可放大、控制和调节预防及治疗性基因疫苗的免疫结果。尽管基因枪有许多令人鼓舞的优点,但由于价格和便利性的障碍,它是否能商业化还不能确定。利用基因法治疗和预防疾病所涉及的安全性对基因疫苗要求更严格。这要有可控的质粒导入系统和组织特异性表达系统。

Delivery of DNA Vaccine

Positive human clinical data using genetic vaccine has proved that genetic vaccine immunization is an effective clinical vaccine modality.Although the precise mechanism of action has not be determined,preclinical studies using jet injection have indicated that direct targeting of resident antigen presenting cells in the skin can significantly enhance immune response.Moreover,positive results with topical delivery of genetic vaccines and exvivo loading of dendritic cells with antigen has suggested that directly targeting antigen presenting cells can amplify,control,and mediate the immune consequences of prophylactic and/or therapeutic gene vaccines.Despite these encouraging advantages of the gene gun,it is unclear whether this technology will become commercially available way due to potential barriers such as cost and convenience.It is clear that safety concerns in using genetic approaches to treat and prevent disease have highlighted the need for strict product requirements for genetic vaccines.It requires controlled plasmid delivery systems and tissue-specific gene expression system. ;