Quantification of clotiazepam in human plasma by gas chromatography-mass spectrometry |
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Authors: | Ahn Sung-Hoon Maeng Han-Joo Koo Tae-Sung Kim Dae-Duk Shim Chang-Koo Chung Suk-Jae |
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Affiliation: | Department of Pharmaceutics, College of Pharmacy, Seoul National University, San 56-1, Shinlim-dong, Kwanak-gu, 151-742, South Korea. |
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Abstract: | An analytical procedure was developed and validated for the quantification of clotiazepam in human plasma. After subjecting plasma samples to solid-phase extraction, the extract was evaporated and the residue re-constituted. An aliquot of the mixture was injected onto a gas chromatography-mass spectrometry system. The detector response was linear for clotiazepam concentrations in the range of 5-200 ng/ml. Intra- and inter-day precision for the assay over the concentration range was below 13.1 and 13.5%, and the accuracy ranged between 99.0-107.9% and 92.4-101.3%, respectively. The drug was found to be stable under various processing conditions used. The method is applicable to human pharmacokinetic studies of clotiazepam. |
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