Total internal reflection with fluorescence correlation spectroscopy: Applications to substrate-supported planar membranes |
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| Authors: | Nancy L. Thompson Xiang Wang Punya Navaratnarajah |
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| Affiliation: | aDepartment of Chemistry, Campus Box 3290, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3290, USA;bDepartment of Biochemistry and Biophysics, Campus Box 7260, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7260, USA |
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| Abstract: | In this paper, the conceptual basis and experimental design of total internal reflection with fluorescence correlation spectroscopy (TIR-FCS) is described. The few applications to date of TIR-FCS to supported membranes are discussed, in addition to a variety of applications not directly involving supported membranes. Methods related, but not technically equivalent, to TIR-FCS are also summarized. Future directions for TIR-FCS are outlined. |
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| Keywords: | Fluorescence microscopy Optical microscopy Evanescent Fluctuation Single molecule Surface binding Diffusion Supported membranes Cell membranes |
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