Optimization, production, and partial characterization of an alkalophilic amylase produced by sponge associated marine bacterium Halobacterium salinarum MMD047 |
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| Authors: | S Shanmughapriya G Seghal Kiran Joseph Selvin R Gandhimathi T Bastin Baskar Aseer Manilal S Sujith |
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| Institution: | (1) Department of Microbiology, Bharathidasan University, Tiruchirappalli, 620024, India;(2) Department of Biotechnology, Bharathidasan University, Tiruchirappalli, 620024, India |
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| Abstract: | An endosymbiont Halobacterium salinarum MMD047, which could produce high yields of amylase, was isolated from marine sponge Fasciospongia cavernosa, collected from the peninsular coast of India. Maximum production of enzyme was obtained in minimal medium supplemented with
1% sucrose. The enzyme was found to be produced constitutively even in the absence of starch. The optimum temperature and
pH for the enzyme production was 40°C and 8.0, respectively. The enzyme exhibited maximum activity in pH range of 6∼10 with
an optimum pH of 9.0. The enzyme was stable at 40°C and the enzyme activity decreased dramatically above 50°C. Based on the
present findings, the enzyme was characterized as relatively heat sensitive and alkalophilic amylase which can be developed
for extensive industrial applications. |
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| Keywords: | amylase characterization extracellular amylase optimization marine amylase sponge bacteria |
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