The orphan nuclear receptor SF-1 is involved in the effect of PCBs,DDT, and DDE on the secretion of steroid hormones and oxytocin from bovine luteal cells during the estrous cycle in vitro |
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Authors: | J. Mlynarczuk M.H. WrobelJ. Kotwica |
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Affiliation: | Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland |
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Abstract: | The orphan receptor steroidogenic factor-1 (SF-1) is involved in the regulation of ovarian steroidogenesis in cows. It is hypothesized that estrogen-like chlorinated compounds might affect SF-1, and thus impair the function of the ovary. Bovine luteal cells from the estrous cycle (Days: 1–5, 6–10, 11–15, and 16–19) were treated for 50 hours with DDT, 1,1-dichloro-2,2-bis(4-chlorophenyl)ethene, 3,3′4,4′-tetrachlorobiphenyl or 2′2′4,4′,5,5′-hexachlorobiphenyl (each at a dose of 10 ng/mL). Luteal cells were also treated with 4-(heptyloxy)phenol (1 × 10−7 M), an SF-1 agonist, and F0160 (1 × 10−6 M), an SF-1 blocker, jointly or separately. The secretion of progesterone and oxytocin and the expression of oxytocin precursor (NP-I/OT) messenger RNA were increased (P < 0.05) by all studied xenobiotics and 4-(heptyloxy)phenol, although they were inhibited (P < 0.05) by F0160. However, the xenobiotics did not affect (P > 0.05) SF-1 messenger RNA expression. In summary, SF-1 is involved in the adverse effect of chlorinated xenobiotics on the regulation of the bovine CL. |
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Keywords: | Chlorinated xenobiotics Progesterone Oxytocin Steroidogenic factor-1 Luteal cell Cow |
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