mir-35 is involved in intestine cell G1/S transition and germ cell proliferation in C. elegans |
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Authors: | Liu Min Liu Pengpeng Zhang Li Cai Qingchun Gao Ge Zhang Wenxia Zhu Zuoyan Liu Dong Fan Qichang |
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Affiliation: | The Education Ministry Key Laboratory of Cell Proliferation and Differentiation and the State Key Laboratory of Bio-Membrane and Membrane Bio-Engineering, Peking University, 5 Summer Palace Road, Beijing 100871, China. |
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Abstract: | MicroRNA (miRNA) regulates gene expression in many cellular events, yet functions of only a few miRNAs are known in C. elegans. We analyzed the function of mir-35-41 unique to the worm, and show here that mir-35 regulates the G1/S transition of intestinal cells and germ cell proliferation. Loss of mir-35 leads to a decrease of nuclei numbers in intestine and distal mitotic gonad, while re-introduction of mir-35 rescues the mutant phenotypes. Genetic analysis indicates that mir-35 may act through Rb/E2F and SCF pathways. Further bioinformatic and functional analyses demonstrate that mir-35 targets evolutionally conserved lin-23 and gld-1. Together, our study reveals a novel function of mir-35 family in cell division regulation. |
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Keywords: | miRNA C. elegans mir-35 G1/S transition germ cell proliferation |
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