激素诱导血清病动物骨坏死模型中的脂类代谢异常

目的初步探讨血清病动物激素性骨坏死的发病机制。方法将新西兰大白兔分为三组,A组联合应用马血清和地塞米松磷酸钠诱导骨坏死模型,B组单纯应用地塞米松磷酸钠,C组应用生理盐水作为对照组,对三组实验兔股骨头进行HE、MRI观察,并对各组兔的血脂、AST和ALT进行检测,分析比较。结果A组出现了典型的股骨头缺血坏死改变,血脂、AST和ALT与另外两组相比差异有显著性。单纯应用激素组和对照组未出现股骨头缺血坏死。结论运用马血清和地塞米松磷酸钠可成功诱导出兔骨坏死模型,血管炎、血脂代谢异常和肝功能损害共同导致股骨头缺血坏死发生,短期应用大剂量激素难以诱导出骨坏死模型。

Abnormal Lipid Metabolism in Serum Disease Animal with Steroid-Induced Osteonecrosis

Objective To explore the pathogenesis of steroid-induced osteonecrosis in serum disease animal. Methods New Zealand rabbits were distributed into three groups randomly. Horse serum and dexamethasone sodium phosphate were used to induce osteonecrosis models in group A, dexamethasone sodium phosphate only in group B and saline in group C. To test and analyze the values of serum lipid, AST and ALT of all rabbits. Rabbits from all groups were observed by MRI to prove osteonecrosis and sacrificed to observe the histopathology of femoral head. Results Typical avascular necrosis of the femoral head happened in group A. The values of serum lipid, AST and ALT are distinctly different from those of other groups of which rabbits did not develop osteonecrosis. Conclusion Horse serum combining dexamethasone sodium phosphate can induce rabbit osteonecrosis model successfully. Vasculitis, abnormal serum lipid metabolism and liver disfunction together result in avascular necrosis of the femoral head. High dose steroid can not induce osteonecrosis in a short time.