Plant regeneration from protoplasts isolated from long-term cell cultures of wheat (Triticum aestivum L.) |
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Authors: | Yin-Fu Chang Wen Chung Wang Colleen Y Warfield Henry T Nguyen Jim R Wong |
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Institution: | (1) Sogetal Inc., 3876 Bay Center Place, 94545 Hayward, California, USA;(2) Department of Agronomy, Horticulture and Entomology, Texas Tech. University, 79409 Lubbock, Texas, USA |
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Abstract: | Summary A friable and fast-growing type of callus was isolated from a long term shoot-competent cell culture of wheat. The suspension cultures established from this callus consisted of small, densely cytoplasmic cells which divided more rapidly but with a lower plant regeneration frequency than the original culture. A high yield of protoplasts was released from suspension cells (2 to 3×107 protoplasts per ml packed cell volume) when treated with enzyme mixtures. The isolated protoplasts divided at a relatively high frequency (20% to 50%) in both liquid and agarose-solidified KM8p medium. Up to 0.21% of the dividing protoplasts continued to divide and form micro-calli. Sixty-eight plants were regenerated from micro-calli, and among the 30 plants which were transplanted to the greenhouse, 3 have survived.Abbreviations BAP
6, enzylaminopurine
- 2,4-D
2,4 dichlorophenoxyacetic acid
- DMSO
dimethylsulfoxide
- FDA
Fluorescein diacetate
- IAA
indole-3-acetic acid
- LS
Linsmaier and Skoog basal medium (1965)
- MES
2, N-morpholino]-ethanesulfonic acid
- MS
Murashige and Skoog basal medium (1962)
- NAA
1, naphthaleneacetic acid
- PCV
packed cell volume |
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