An anion-selective channel from the Pseudomonas aeruginosa outer membrane |
| |
Authors: | Roland Benz Michaela Gimple Keith Poole Robert EW Hancock |
| |
Abstract: | Protein P from Pseudomonas aeruginosa outer membrane was reconstituted in lipid bilayer membranes from diphytanoylphosphatidylcholine. The reconstitution resulted in the formation of anion-selective channels with a conductance of 160 pS for 0.1 M chloride solution. The channels were at least 100-times more selective for anions than for cations as judged from zero-current membrane potentials. The single-channel conductance was dependent on the size of the different anions and saturated at higher salt concentrations suggesting single ion occupancy of the protein P channel. |
| |
Keywords: | Outer membrane Anion selectivity Channel formation Lipid bilayer Membrane reconstitution (Ps aeruginosa) |
本文献已被 ScienceDirect 等数据库收录! |