Tanshinone IIA and tanshinone I production by Trichoderma atroviride D16, an endophytic fungus in Salvia miltiorrhiza |
| |
Authors: | Ming Qianliang Han Ting Li Wenchao Zhang Qiaoyan Zhang Hong Zheng Chengjian Huang Fang Rahman Khalid Qin Luping |
| |
Institution: | Department of Pharmacognosy School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, PR China. |
| |
Abstract: | In this study the isolation of an endophytic fungus from the root of the medicinal herb Salvia miltiorrhiza Bunge is reported for the first time. The fungus produced tanshinone I and tanshinone IIA in rich mycological medium (potato dextrose broth) under shake flask and bench scale fermentation conditions. The fungus was identified as Trichoderma atroviride by its morphology and authenticated by ITS analysis (ITS1 and ITS2 regions and the intervening 5.8S rDNA region). Tanshinone I and tanshinone IIA were identified by HPLC and LC-HRMS/MS and confirmed through comparison with authentic standards. This endophytic fungus has significant scientific and industrial potential to meet the pharmaceutical demands for tanshinone I and tanshinone IIA in a cost-effective, easily accessible and reproducible way. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|