Transformation of cauliflower (Brassica oleracea L. var. botrytis) — an experimental survey |
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| Authors: | K Eimert F Siegemund |
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| Institution: | (1) Dept. of Genetics, Martin Luther University, Domplatz 1, 0-4020 Halle/S., Germany |
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| Abstract: | The paper compares different approaches for the genetic transformation of cauliflower (Agrobacterium-mediated, PEG-mediated and/or electroporation). Transient expression of the neomycin phosphotransferase II (NPTII) gene could be detected after direct gene transfer. Stable transformation was achieved using both Agrobacterium-mediated and direct gene transfer. Expression as well as incorporation of the NPTII sequence could be demonstrated. |
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| Keywords: | Agrobacterium tumefaciens Brassica oleracea electroporation genetic transformation NPTII |
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