A simple, versatile, nondisruptive method for the isolation of morphologically and chemicaly pure basement membranes from several tissues. |
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Authors: | E Meezan J T Hjelle K Brendel |
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Affiliation: | Department of Pharmacology, University of Arizona College of Medicine, Tucson, Arizona 85724, USA;Department of Anatomy, University of Arizona College of Medicine, Tucson, Arizona 85724, USA |
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Abstract: | A simple procedure has been developed for the isolation of ultrastructurally pure, intact basement membranes from bovine retinal and brain blood vessels, rabbit renal tubules and rat renal glomeruli. By this procedure, cell membranes and intracellular materials are selectively solubilized with 4% sodium deoxycholate to yield morphologically and chemically intact basement membrane preparations. Therefore, this method appears to be a versatile, nondisruptive procedure for the isolation and characterization of basement membranes from a variety of tissues. Its applicability has been demonstrated by the preparation for the first time of isolated basement membranes from non-renal mammalian blood vessels. |
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Keywords: | To whom all reprint requests should be directed. |
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