Ageing-related changes in Mycoplasma canadense membranes |
| |
Authors: | Gtaciela E Muñoz CP Sotomayor |
| |
Institution: | Laboratorio de Genética Molecular, Instituto de Biologia;Laboratorio de Espectroscopia Molecular lnstituto de Quimica, Facultad de Ciencias Básicas y Matemáticas, Universidad Católica de Valparaiso, Chile |
| |
Abstract: | Fluidity and composition of cell membranes during progression of Mycoplasma canadense cultures grown in a serum-free medium was assessed. The fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene at 25°C of intact cells and liposomes in the exponential and stationary phases of growth was compared. A decrease in fluidity and an increase in the ratio of saturated to unsaturated fatty acids was detected in cell membranes on ageing. Nevertheless, membrane density remained unaltered although the molar ratio of cholesterol to phospholipids decreased. It is proposed that the increase in lipid order is primarily due to the increase in the ratio of saturated to unsaturated membrane fatty acids, being the diminished molar ratio of cholesterol to phospholipids involved in the reduced unsaturated fatty acid uptake. |
| |
Keywords: | |
|
|