Isolation and characterization of a sporeless mutant in <Emphasis Type="Italic">Pleurotus eryngii</Emphasis> |
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Authors: | Y Obatake S Murakami T Matsumoto Y Fukumasa-Nakai |
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Institution: | (1) Nara Forest Research Institute, 1 Kibi, Takatori, Nara 635-0133, Japan Tel. +81-744-52-2380; Fax +81-744-52-4400 obatakey@nararinshi.pref.nara.jp, JP;(2) The Tottori Mycological Institute, Tottori, Japan, JP |
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Abstract: | A sporeless mutant dikaryon, completely defective in sporulation, was isolated from mycelial protoplasts of Pleurotus eryngii mutagenized by UV irradiation. Newly established dikaryons between one component monokaryon from the mutant, and 12 different
wild type monokaryons from 3 other wild type dikaryons, all exhibited the sporeless phenotype, whereas those between the other
monokaryon and the same wild type monokaryons all produced normal fruiting bodies. These results indicated that the sporeless
mutation was induced in one of two nuclei of the mutant and was dominant. In the wild type basidia, the pattern of nuclear
behavior during sporulation corresponded to the pattern C nuclear behavior as defined by Duncan and Galbraith. Cytological
observation revealed that in the sporeless mutant meiosis was blocked at the meta-anaphase I in most basidia and hence basidiospores
and sterigmata were not produced. Although fruiting bodies of the sporeless mutant showed a somewhat leaning growth, their
gross morphology and its fruiting body productivity were comparable to that of the original wild type strain. Based on these
results, it was considered that the sporeless mutant could serve as a potential material in breeding of sporeless P. eryngii commercial strains.
Received: September 5, 2002 / Accepted: October 16, 2002
Acknowledgments We are grateful to Mrs. Motoe Masuda for her skillful technical assistance.
Contribution no. 358 from the Tottori Mycological Institute
Correspondence to:Y. Obatake |
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Keywords: | Nuclear behavior Pleurotus eryngii Sporeless mutation |
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