非小细胞肺癌组织CENPF、KLF4表达与上皮-间质转化和预后的关系研究

摘要 目的：探讨非小细胞肺癌（NSCLC）组织中着丝粒蛋白F（CENPF）、Krüppel样因子4（KLF4）表达与上皮-间质转化（EMT）和预后的关系。方法：选取2017年1月至2019年12月期间于泰州市第四人民医院行手术切除的120例NSCLC患者的癌组织和距癌组织5cm癌旁组织标本，采用免疫组织化学法和免疫印迹法检测癌组织和癌旁组织中CENPF、KLF4及ETM相关标志物E-钙粘蛋白（E-cadherin）、波形蛋白（Vimentin）]的阳性表达率和表达量。采用Pearson检验分析CENPF、KLF4与EMT相关标志物的相关性，并分析CENPF、KLF4表达与NSCLC患者预后的关系。结果：NSCLC癌组织中CENPF、Vimentin的阳性表达率显著高于癌旁组织（均P<0.05），而KLF4、E-cadherin的阳性表达率均显著低于癌旁组织（均P<0.05）。NSCLC癌组织中CENPF与E-cadherin呈负相关，与Vimentin呈正相关（P<0.05）；而KLF4与E-cadherin呈正相关，与Vimentin呈负相关（P<0.05）。NSCLC癌组织中CENPF、KLF4的阳性表达率与TNM分期和淋巴结转移有关（均P<0.05）。入组患者3年无病生存率（DFS）为60.00%。CENPF阳性表达的NSCLC患者3年DFS显著低于CENPF阴性表达患者（56.25% vs 75.00%，P=0.014），KLF4阳性表达的NSCLC患者3年DFS显著高于KLF4阴性表达患者（68.75% vs 54.17%， P=0.048）。结论：CENPF的高表达及KLF4的低表达可促进NSCLC的EMT发生、进展，并导致患者预后不良，CENPF和KLF4可辅助预测NSCLC患者的预后。

Relationship Study between the Expression of CENPF and KLF4 and Epithelial-Mesenchymal Transformation and Prognosis in Non-Small Cell Lung Cancer Tissue

ABSTRACT Objective: To investigate the relationship between the expression of centromeric protein F (CENPF) and Krüppel-like factor 4(KLF4) and epithelial-mesenchymal transformation (EMT) and prognosis in non-small cell lung cancer (NSCLC) tissue. Methods: Cancer tissue samples and paracancer tissue samples 5 cm away from carcinoma tissue of 120 NSCLC patients who underwent surgical resection in Taizhou Fourth People''s Hospital from January 2017 to December 2019 were selected. The positive expressions rate and amount of CENPF, KLF4 and ETM-related markers E-cadherin and Vimentin] in cancer tissue and paracancer tissue were detected by immunohistochemistry and Western blotting methods. Pearson test was used to analyze the correlation between CENPF, KLF4 and EMT-related markers, and the relationship between the expression of CENPF and KLF4 and the prognosis of NSCLC patients were analyzed. Results: The positive expression rates of CENPF and Vimentin in the NSCLC cancer tissue were significantly higher than those in paracancer tissue (all P<0.05), while the positive expression rates of KLF4 and E-cadherin were significantly lower than those in the paracancer tissue (all P<0.05). CENPF in the NSCLC cancer tissue was negatively correlated with E-cadherin, and positively correlated with Vimentin (P<0.05). KLF4 was positively correlated with E-cadherin, and negatively correlated with Vimentin (P<0.05). The positive expressions rate of CENPF and KLF4 in NSCLC cancer tissue were associated with TNM staging and lymph node metastasis (all P<0.05). The 3-year disease-free survival rate (DFS) of enrolled patients was 60.00%. The 3-year DFS of NSCLC patients CENPF positive expression were significantly lower than those with CENPF negative expression patients (56.25% vs 75.00%, P=0.014). The 3-year DFS of NSCLC patients with KLF4 positive expression were significantly higher than those with KLF4 negative expression (68.75% vs 54.17%, P=0.048). Conclusion: The high expression of CENPF and low expression of KLF4 can promote the occurrence and progression of EMT in NSCLC, and lead to poor prognosis of patients. CENPF and KLF4 can assist in predicting the prognosis of patients with NSCLC.