杨梅素对高脂喂养小鼠代谢及自发活动节律的影响研究

摘要 目的：探讨杨梅素对高脂喂养小鼠代谢情况及自发活动节律的影响。方法：6周龄清洁级C57BL/6雄性小鼠15只，随机分为普通饲料组（CON）、高脂饲料组（HFD）、高脂饲料+杨梅素组100 mg /（kg?d）组（HFD+MYR）。从干预第10周开始使用Clocklab生物节律采集分析系统记录三组小鼠自发活动数据。干预第13周结束，检测三组小鼠体重、血脂数据。结果：与CON组相比，HFD组体重、甘油三酯、总胆固醇、低密度脂蛋白均显著升高（P<0.001），高密度脂蛋白显著降低（P<0.001），活动峰值时相（Activity phase）显著后移（P<0.001），自发活动量中值（Activity mesor）和总自发活动量（Total counts）明显增加（P<0.05），HFD+MYR组体重和低密度脂蛋白无明显变化（P>0.05），甘油三酯、总胆固醇均显著升高（P< 0.01），高密度脂蛋白显著降低（P<0.01），活动峰值时相显著后移（P<0.001），自发活动量中值和总自发活动量无明显变化（P>0.05）。与HFD组相比，HFD+MYR组体重、甘油三酯、总胆固醇、低密度脂蛋白明显降低（P<0.05），高密度脂蛋白明显升高（P<0.05），活动峰值时相明显前移（P<0.05），自发活动量中值和总自发活动量明显减少（P<0.05）。结论：杨梅素可改善高脂喂养小鼠的代谢状态及减轻小鼠自发活动节律紊乱。

Effect of Myricetin on Metabolism and Circadian Rhythm of Spontaneous Activity in High-fat-diet Mice

ABSTRACT Objective: To explore the effect of myricetin on metabolism and biological rhythm of spontaneous activity in high-fat-diet mice. Methods: Fifteen 6-week-old clean grade C57BL/6 male mice were randomly divided into normal diet group (CON), high-fat diet group (HFD) and high-fat diet + myricetin (100 mg/(kg?d) ] group (HFD+MYR). From the 10th week of intervention, the spontaneous activity data of the three groups of mice were recorded with the ClockLab biorhythm analysis system. At the end of the 13th week of intervention, the body weight and blood lipid levels of the mice in the three groups were measured. Results: Compared with the CON group, the weight, triglycerides, total cholesterol, and low-density lipoprotein were significantly increased (P<0.001) in the HFD group, while high-density lipoprotein was significantly decreased (P<0.001). The activity phase of the HFD group was significantly delayed (P<0.001), and the activity mesor and total counts were significantly increased (P<0.05). In the HFD+MYR group, there were no significant changes in the body weight and low-density lipoprotein (P>0.05), while triglycerides and total cholesterol were significantly increased (P<0.01), and high-density lipoprotein was significantly decreased(P<0.01), the activity phase was significantly delayed (P<0.001), and there were no significant changes in the activity mesor and total counts (P>0.05) compared with CON group. Compared with the HFD group, the body weight, triglycerides, total cholesterol, and low-density lipoprotein were significantly decreased (P<0.05) in the HFD+MYR group, while high-density lipoprotein was significantly increased (P<0.05). The activity phase of the HFD+MYR group was significantly advanced(P<0.05), and the activity mesor and total counts were significantly reduced (P<0.05). Conclusion: Myricetin can improve the metabolic state and relieve the biological rhythm disorder of spontaneous activity in high-fat-fed mice.