Modeling the highly specific ribotoxin recognition of ribosomes |
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Authors: | García-Mayoral Flor García-Ortega Lucía Alvarez-García Elisa Bruix Marta Gavilanes José G del Pozo Alvaro Martínez |
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Institution: | Departamento de Bioquímica y Biología Molecular I, Facultad de Química, Universidad Complutense, 28040 Madrid, Spain. |
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Abstract: | The three-dimensional structures of the alpha-sarcin ribotoxin and its delta(7-22) deletion mutant, both complexed with a 20-mer oligonucleotide mimicking the sarcin/ricin loop (SRL) of the ribosome, have been docked into the structure of the Halobacterium marismortui ribosome by fitting the nucleotide atomic coordinates into those of the ribosomal SRL. This study has revealed that two regions of the ribotoxin, residues 11-16 and 84-85, contact the ribosomal proteins L14 (residues 99-105) and L6 (residues 88-92), respectively. The first of these two ribotoxin regions appears to be crucial for its specific ribosome recognition. |
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Keywords: | RIP ribosome-inactivating proteins SRL sarcin/ricin loop |
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