Reduced levels of rat lens antioxidant vitamins upon in vitro UVB irradiation

Ultraviolet (UV) radiation is one of the major risk factors of cataractogenesis. UV radiation induced damage to the eye lens is believed to be mediated through reactive oxygen species. Antioxidant defense systems, enzymatic and non-enzymatic, resist this damage. In the present study, the levels of rat lens endogenous antioxidants, L-ascorbic acid, alpha-tocopherol and beta-carotene, have been determined by HPLC upon in vitro UVB irradiation. UVB irradiation for 24 h (300 nm; 100 μW/cm(2)) of three months old rat lens suspended in RPMI medium, leads to 69-89% decrease in endogenous levels of these antioxidants. The addition of ascorbic acid (2 mM), alpha-tocopherol (2.5 μM) or beta-carotene (10 μM), separately to the medium during irradiation significantly prevented the decrease in their endogenous levels, thereby suggesting a protective role for these antioxidant micronutrients against photodamage to the eye lens.