Staining of demineralized cartilage |
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| Authors: | R W Jubb F M Eggert |
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| Institution: | (1) Department of Rheumatology, Westminster Hospital, SW1P 2AP London, England;(2) Department of Oral Medicine and Periodontology, Dental School, The London Hospital, E1 2AD London, England;(3) Present address: Department of Periodontology, Faculty of Dentistry, The University of Alberta, T6G 2N8 Edmonton, Alberta, Canada |
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| Abstract: | Summary Safranin O in the orthochromatic form stains articular cartilage proteoglycan quantitatively in histological sections of demineralized cartilage. This was shown by scanning microdensitometry of stained sections of undemineralized and demineralized articular cartilage and by biochemical analysis of 35S labelled cartilage subjected to demineralization. In constrast, Alcian Blue staining is affected by unknown factors other than simply the amount of proteoglycan present. Alcoholic formalin fixes articular cartilage proteoglycan more successfully than formol Zenker for subsequent rapid demineralization. Alcoholic formalin does not preserve cellular appearance as well as formol Zenker. Staining of articular cartilage with PAS appears unaffected by demineralization. |
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