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Possible role of transient electric fields in freezing-induced membrane destabilization |
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| Authors: | Peter L Steponkus Darryl G Stout Joe Wolfe Richard V E Lovelace |
| Institution: | (1) Department of Agronomy, Cornell University, Ithaca, New York;(2) Range Research Station, Agriculture Canada, Kamloops, B.C., Canada;(3) School of Physics, University of N.S.W., Kensington, N.S.W., Australia;(4) Department of Applied Physics, Cornell University, Ithaca, New York |
| Abstract: | Summary Evidence is presented to support the hypothesis that electrical potentials generated during the freezing of aqueous solutions (the Workman-Reynolds effect) may contribute to the destabilization of the plasma membrane and cryoinjury of isolated protoplasts. Specifically. (1) electric potential diffrences of sufficient magnitude to cause lysis of the plasma membrane occur during the rapid freezing of isolated protoplasts suspended in sorbitol: (2) survival of protoplasts is inversely correlated with the magnitude of the potential difference and (3) cold acclimation increases the stability of the plasma membrane to applied electric fields. A discussion is given of the different physical phenomena though to be involved in the Workman-Reynolds effect. The basis equations for these phenomena are outlined. |
| Keywords: | isolated protoplasts plasma membrane freezing injury freeze-induced electrical transients Workmann-Reynolds effect |
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