Hepatocellular Carcinoma Cells Deteriorate the Biophysical Properties of Dendritic Cells |
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Authors: | Zhu Zeng Weijuan Yao Xiaofeng Xu Guoqiang Xu Jinhua Long Xianwei Wang Zongyao Wen Shu Chien |
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Institution: | (1) Department of Biophysics, Health Science Center, Peking University, Beijing, 100191, People’s Republic of China;(2) Guiyang Medical College, Guiyang, Guizhou Province, China;(3) Departments of Bioengineering and Medicine, University of California, La Jolla, San Diego, CA 92093-0412, USA |
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Abstract: | Dendritic cells (DCs) are potent antigen-presenting cells and induce antigen-specific immune responses in the organism. The
dysfunction of DCs has been implicated in tumor-bearing host. In order to elucidate the effects of tumor microenvironment
on the functions of DCs from interdisciplinary aspects, we characterized the biophysical properties of DCs co-cultured with
hepatocellular carcinoma cells (HCC). The results showed that the biophysical characteristics of immature and mature DCs were
severely impaired by HCC compared with those under normal conditions, including the increased osmotic fragilities, decreased
cell membrane fluidities, increased membrane viscoelastic properties, dysfunction and increased expression of cytoskeleton
protein F-actin, as well as the deteriorated transendothelium migration. The impaired biophysical properties of DCs may be
one of many aspects of the immune escape mechanisms of tumors. These results are clinically and instructionally significant
with regard to how to enhance efficiency of the anti-tumor therapy based on DCs.
Zhu Zeng and Weijuan Yao contributed equally to this work. |
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Keywords: | Dendritic cells Biophysics Hepatocellular carcinoma Microenvironment |
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