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A yeast bioassay for T-2 toxin
Authors:KT Schappert  GG Khachatourians
Institution:Department of Applied Microbiology and Food Science, and the Toxicology Group, University of Saskatchewan, Saskatoon, Saskatchewan S7N OWO Canada
Abstract:A disk diffusion type bioassay was developed for T-2 toxin using the yeast Kluyveromyces fragilis. The lower limit of detection for this in 0.2 μg of T-2 toxin. The growth of this yeast was sensitive to other trichothecenes such as verrucarin A (0.01 μg). Aflatoxin B1 (50 μg) and zearalanone (20 μg) did not inhibit the growth of this yeast.
Keywords:Bioassay  T-2 toxin  Trichothecenes  Verrucarin A  Yeast  Zearlenone
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