A new culturing method for production of filamentous hemagglutinin of Bordetella pertussis |
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| Authors: | Atsushi Imaizumi Yoji Suzuki Akihiro Ginnaga Shin Sakuma Yuji Sato |
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| Institution: | 1. Teijin Institute for Biomedical Research, Hino, Tokyo 191, Japan;2. Chemo-Sero Therapeutic Research Institute, Shimizu-cho, Kumamoto 860, Japan;3. 1st Department of Bacteriology, National Institute of Health, Kamiosaki, Shinagawa-ku, Tokyo 141 Japan |
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| Abstract: | The addition of cyclodextrins, especially heptakis (2,6-O-dimethyl)β-cyclodextrin (MeβCD), to synthetic medium, such as Stainer-Scholte medium (D.W. Stainer and M.J. Scholte, J. Gen. Microbiol. 63: 211–220) greatly stimulated the production of filamentous hemagglutinin (F-HA) by Bordetella pertussis Tohama phase I under shaking conditions. Purified F-HA from this culturing system was demonstrated immunochemically and morphologically to be identical with that from static culture. |
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| Keywords: | Cyclodextrin ELISA Filamentous hemagglutinin Pertussis toxin Pertussis vaccine |
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