Electrochemical changes in media due to microbial |
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Authors: | Ruth Firstenberg-Eden Joseph Zindulis |
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Institution: | Bactomatic, A Division of Medical Technology Corporation, P.O. Box 3103, Princeton, NJ 08540 U.S.A. |
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Abstract: | Microbial metabolism affected the electrical impedance parameters of a two terminal-measuring cell-containing growth media. The relationship between microbial growth and relative changes in both The capacitive and resistive parts of impedance was examined. Both components of impedance were shown to be indicative of bacterial growth. In low conductivity media the change in the conductance of the media (Gsol) clearly correlated to bacterial growth. In more conductive media the relative changes in Gsol were smaller, and in these media measurements of the changes of polarization capacitance (Cpol) were useful for monitoring bacterial growth.Yeast growth in two media resulted in large changes in Cpol (20–100%) while the changes in Gsol were very small (1–4%). This result indicated that, for some combinations of microorganisms and media, measuring Cpol might be preferable over Gsol for the detection of microbial growth.Microbial metabolism resulted in a change of 2–2.5 units in pH. This pH change resulted in a 40% change in Cpol but less than a 14% change in Gsol. |
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Keywords: | Capacitance Conductance Impedance Yeast monitoring |
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