Kinetic properties of de-repressible alkaline phosphatase (EC 3.1.3.1) from Neurospora crassa |
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| Authors: | M. S. Palma S. W. Han A. Rossi |
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| Affiliation: | (1) Departamento de Biologia, IB-UNESP, 13500 Rio Claro, SP., Brazil;(2) Departamento de Bioquimica, IB-UNESP, 13500 Rio Claro, SP., Brazil;(3) Departamento de Quimica, FFCLRP-USP, Au Bandeirantes 3900, 14049 Ribeirão Preto, SP., Brazil |
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| Abstract: | De-repressible alkaline phosphatase from N. crassa shows inhibition by PNP-P and a hyperbolic mixed-type inhibition by Pi. Both increasing concentrations of Pi and decreases in assay pH abolished inhibition by the substrate. Also, Pi promoted polymerization of the enzyme molecule, whose effect may account for the inhibitory behaviour shown by the enzyme in the presence of low Pi concentrations. |
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