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Immunohistochemical identification of Purkinje fibers and transitional cells in a terminal portion of the impulse-conducting system of porcine heart |
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| Authors: | Hirotaka Toshimori Kiyotaka Toshimori Chikayoshi ōura Hisayuki Matsuo Shigeru Matsukura |
| Institution: | (1) Department of Anatomy, Miyazaki Medical College, Miyazaki, Japan;(2) Miyazaki Prefectural Hospital, Kita-Takamatsu, Miyazaki, Japan;(3) Department of Internal Medicine, Miyazaki Medical College, Miyazaki, Japan;(4) Department of Biochemistry, Miyazaki Medical College, Miyazaki, Japan;(5) Department of Internal Medicine, Miyazaki Medical College, 889-16 Miyazaki, Japan |
| Abstract: | Summary The ultrastructure of porcine ventricular tissue was studied by electron microscopy and immunocytochemical techniques. Electron-dense specific granules were found in both Purkinje fibers and transitional cells in the ventricular walls, and were positively stained by the immunogold staining method using an antiserum against atrial natriuretic polypeptide (ANP). This suggests that both the Purkinje fibers and transitional cells display the same specific granules as atrial cardiocytes containing ANP. These results demonstrate that Purkinje fibers and two types of transitional cells, in addition to the ordinary ventricular cardiocytes, can be identified in porcine ventricular wall tissue. |
| Keywords: | Heart Atrial-specific granules Atrial natriuretic polypeptide (ANP) Purkinje fiber Immunohistochemistry Impulse-conducting system Swine (Sus scrofa domestica) |
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