Isolation and characterization of 15 microsatellite loci from Caridina gracilipes (Atyidae, Decapoda) |
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Authors: | Chiao-Chuan Han Cheng-Sheng Chang Tzen-Yuh Chiang Ping-Han Chung Hung-Du Lin |
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Institution: | (1) National Museum of Marine Biology and Aquarium, Checheng, Pingtung, 944, Taiwan;(2) Institute of Marine Biodiversity and Evolutionary Biology, National Dong Hwa University, Checheng, Pingtung, 944, Taiwan;(3) Department of Life Sciences, National Cheng Kung University, Tainan, 701, Taiwan |
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Abstract: | Caridina gracilipes, an atyid shrimp of Taiwan, inhabits the fresh and brackish waters. Its life history can be classified into amphidromous
and landlocked types. Recent human’s transportation has caused concerns of genetic contamination between these long evolved
lineages and ecological instabilities. Molecular markers with high sensitivities are required for detecting the possible introgression
due to human disturbance. In the study, we described the development of 15 polymorphic microsatellite loci in C. gracilipes for genetic and ecological studies. These new markers were tested in 20 individuals of three populations. The number of alleles
ranged from 6 to 17. Expected (H
E) and observed (H
O) heterozygosities ranged from 0.653 to 0.954, averaged at 0.865, and from 0.000 to 0.4, averaged at 0.146, respectively.
All loci are significantly deviated from Hardy–Weinberg expectations due to the heterozygote deficiency. Significant genetic
differentiation among populations was detected based on F
ST values, which were estimated from 0.82 to 0.88.
Chiao-Chuan Han and Cheng-Sheng Chang are contributed equally to this work. |
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Keywords: | Caridina gracilipes Heterozygosity Introgression Microsatellite PIMA RAPD-PCR enrichment |
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