Mechanism of chain initiation by dextransucrase: Absence of terminal ‘sucrose’ linkage in newly synthesized dextran chains |
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Authors: | Veena K Parnaik Mayer Robert M |
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Institution: | Department of Chemistry, The Ohio State University, Columbus, OH 43210, USA |
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Abstract: | Dextran was synthesized using dextransucrase from Streptococus sanguis 10558 and (F)-14C]sucrose as substrate to test the possibility that sucrose may be the initial acceptor for glucose. If sucrose is the initial acceptor, then dextran chains should have 14C] fructose in a terminal ‘sucrose’ linkage which can be cleaved under mild conditions. Although incorporation of 14C]fructose into dextran was observed, the label was not released by mild hydrolysis, indicating that sucrose is not the initiator for dextran synthesis. Incorporation of 14C]fructose into dextran might represent its ability to act as an acceptor, as suggested by the isolation of leucrose as a by-product in the reaction. |
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Keywords: | Dextransucrase Polysaccharide synthesis Chain initiation |
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