[3H]Muscimol Binding Site on Purified Benzodiazepine Receptor |
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Authors: | C. Martini T. Rigacci A. Lucacchini |
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Affiliation: | Istituto Policattedra di Discipline Biologiche, Universitádi Pisa, Pisa, Italy |
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Abstract: | The presence of a [3H]muscimol binding site on the purified benzodiazepine receptor was demonstrated. The purified protein was apparently homogeneous as shown by sodium dodecyl sulfate polyacrylamide gel electrophoresis (stained with silver), with a molecular weight of 60,000 +/- 3000. The benzodiazepine binding sites were characterized as being of the central type and the [3H]flunitrazepam binding was enhanced by GABA. This activation was antagonized by bicuculline. [3H]Muscimol specifically binds to the benzodiazepine receptor. The Scatchard plot indicates a Kd of 23 nM and the ratio [3H]flunitrazepam/[3H]muscimol is approximately unity. |
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Keywords: | Benzodiazepine binding sites γ-Aminobutyric acid binding sites Muscimol |
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