STUDIES WITH MURINE LPC-1 PLASMACYTOMA USING [6-14C]ARGININE |
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Authors: | R. L. Humphrey S. M. Trivedi Carmelita G. Frondoza |
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Affiliation: | Immunochemistry Laboratory, Oncology Center, The Johns Hopkins University School of Medicine, Baltimore, Maryland, U.S.A. |
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Abstract: | [6-14C]Arginine ([6-14C]Arg) was used as an in vivo pulse label to study BALB/c murine LPC-1 plasmacytoma synthesis and secretion of its tumour-associated M component (IgG2a, k). With this isotope, an eight- to ten-fold enhancement in the labelling of the γ globulin region and ten-fold reduction in the albumin labelling were observed. Production and secretion of the M component was detected (within 30 min) after cell transfer. Only mice which received tumour cells showed significant labelling in the γ globulin region 24 hr after isotope injection. The labelling behaviour of the tumour M component correlated with the administered cell dose. The peak heights of radioactivity in the γ region increased with increments in cell number. When the percentage radioactivity diverted into M component was plotted as a function of cell dose, a linear relationship was noted. This study demonstrates the feasibility of using [6-14C]Arg as a tool to follow the newly synthesized tumour-associated protein, and provides a means of estimating tumour cell number. |
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