Protein synthesis in tissues cultured from the bovine hoof |
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Authors: | K. A. K. Hendry M. J. Lancelott C. H. Knight S. A. Kempson C. J. Wilde |
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Affiliation: | (1) Department of Anatomy, Showa University School of Medicine, 142 Shinagawa Tokyo, Japan;(2) School of Fisheries Sciences, Kitasato University, Sanriku, 022-01 Iwate, Japan;(3) Department of Fisheries, Faculty of Agriculture, The University of Tokyo, 113 Bunkyo, Tokyo, Japan;(4) The Nikko Branch, National Research Institute of Aquaculture, 321-16 Nikko, Tochigi, Japan |
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Abstract: | Salmon gonadotropin (GTH II) is a heterodimeric glycoprotein hormone ( and II subunits), serving as a maturational GTH, and is produced in a specific gonadotropic cell-type (GTH II-cells) containing small granules and large globules. In trout GTH II-cells, double immunolabeling for the - and II-subunits shows that colocalization of the - and II-immunolabeling is confined to the small granules, indicating storage of functional GTH II. On the other hand, -immunolabeling is absent in the large globules, even though II labeling is abundant throughout the period of seasonal gametogenesis. The -specific antiserum recognizes the intact -subunit as well as the reduced and deglycosylated -subunits by immunoblotting. These results indicate that an accumulation of the II-subunit is specifically generated in the large globules of these cells. In fact, with sexual maturity, the quantity of II-subunits becomes elevated in the trout pituitary due to a marked increase in GTH II-cells containing many large globules. However, the derivation and function of the large globules and the fate of their contained II-subunits remains unknown. |
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Keywords: | Pituitary gland Gonadotropin Subunits Gonadotropes Immunocytochemistry Immunoblotting Oncorhynchus mykiss (Teleostei) |
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