Molecular relationships of degradative plasmids determined by in situ hybridisation of their endonuclease-generated fragments |
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| Authors: | A L Heinaru C J Duggleby P Broda |
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| Affiliation: | (1) Department of Molecular Biology, King's Buildings, Edinburgh University, Mayfield Road, EH9 3JR Edinburgh, Scotland;(2) Present address: Department of Genetics and Cytology, Tartu State University, 40 Mitchurini Street, 202400 Tartu, Estonian S.S.R., USSR |
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| Abstract: | Summary Plasmid inter-relationships were studied by hybridisation of a radioactively labelled DNA probe to endonuclease-derived fragmentation patterns of plasmids bound to a nitrocellulose filter. The degradative plasmids SAL and NAH were found to be very closely related, but probably one did not give rise to the other by just a single deletion or insertion. Relationships between SAL and other degradative plasmids are complex; substantial homology was found with TOL and other plasmids encoding toluate dissimilation and significant homology was found with OCT. |
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