Morphogenetic responses obtained from a variety of somatic explant tissues of data palm |
| |
Authors: | Abdelouahhab Zaid Brent Tisserat |
| |
Institution: | (1) Agricultural Research Service, Fruit and Vegetable Chemistry Laboratory, U.S. Department of Agriculture, 91106 Pasadena, CA, USA |
| |
Abstract: | Shoot tip, bud, leaf, stem and root explants from bearing trees, offshoots, seedlings, and asexual plantlets ofPhoenix dactylifera L. were cultured on modified Murashige and Skoog nutrient media containing 3 g/l activated charcoal, 100 mg/l 2,4-dichlorophenoxyacetic acid, 3 mg/l
N
6-(Δ2-isopentyl)adenine to obtain callus. Differential morphogenetic responses were obtained from calli dependent on the explant
type and parent source. Subcultured shoot tips and leafy lateral buds callus on nutrient media devoid of charcoal and supplemented
with 0.1 mg/l α-naphthaleneacetic acid (NAA) produced adventitious plantlets. Subcultured leaf calli produced roots only. Root callus failed
to exhibit any morphogenetic response upon subculturing. Undifferentiated non-leafy buds and stem tissues did not give rise
to callus, regardless of the parent source. Generally, the best callus and embryogenetic responses from explants were obtained
from seedling and plantlet parent sources. Similarly, organogenetic responses such as root formation and shoot development
from shoot tips cultured on media containing 10 mg/l NAA were also related to the parent explant source.
Mention of a trademark or proprietary product in the paper does not constitute a guarantee or warranty of the product by the
U.S. Department of Agriculture and does not imply its approval to the exclusion of other products that may also be available. |
| |
Keywords: | Arecaceae Callus Embryogenesis Lateral bud Phoenix dactylifera L Shoot tip |
本文献已被 SpringerLink 等数据库收录! |
|